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验证数据展示

  • WB analysis of mouse brain using 18738-1-AP

    WB analysis of mouse brain using 18738-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18738-1-AP (MAPK4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 18738-1-AP

    IHC staining of human heart using 18738-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18738-1-AP (MAPK4 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with MAPK4 antibody included
  • IHC staining of human heart using 18738-1-AP

    IHC staining of human heart using 18738-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18738-1-AP (MAPK4 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with MAPK4 antibody included

MAPK4 Polyclonal antibody

MAPK4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18738-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ERK 4, ERK3, Erk4, MAP kinase 4, MAP kinase isoform p63, MAPK 4, MAPK4, p63 MAPK, p63MAPK, PRKM4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MAPK4 Ready-To-Use IHC Kit. MAPK4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18738-1-AP targets MAPK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase 4
别名 ERK 4, ERK3, Erk4, MAP kinase 4, MAP kinase isoform p63, MAPK 4, MAPK4, p63 MAPK, p63MAPK, PRKM4
计算分子量 66 kDa
观测分子量 68 kDa
GenBank蛋白编号BC117216
基因名称 MAPK4
Gene ID (NCBI) 5596
RRIDAB_2878559
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31152
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAPK4 antibody 18738-1-APDownload protocol
IHC protocol for MAPK4 antibody 18738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols