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ERK5 Polyclonal antibody

ERK5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11164-1-AP

Print datasheet

Synonyms

ERK 5, MAPK7, MAPK 7, Extracellular signal-regulated kinase 5, ERK-5

验证数据展示

  • WB analysis of SKOV-3 using 11164-1-AP

    WB analysis of SKOV-3 using 11164-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11164-1-AP (ERK5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 11164-1-AP

    IHC staining of human stomach using 11164-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11164-1-AP (ERK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAPK7 antibody included

经过测试的应用

Positive WB detected inSKOV-3 cells, HEK-293 cells, mouse heart tissue, mouse testis tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MAPK7 Ready-To-Use IHC Kit. MAPK7 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11164-1-AP targets ERK5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ERK5 fusion protein Ag1648 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase 7
Synonyms ERK 5, MAPK7, MAPK 7, Extracellular signal-regulated kinase 5, ERK-5
Calculated Molecular Weight 89 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC009963
Gene Symbol MAPK7
Gene ID (NCBI) 5598
RRIDAB_2877749
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13164
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ERK5 antibody 11164-1-APDownload protocol
IHC protocol for ERK5 antibody 11164-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.

Authors - Xiulei Mo
View Article