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  • Featured Product
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EB1 Polyclonal antibody

EB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17717-1-AP

Print datasheet

Synonyms

MAPRE1, Microtubule-associated protein RP/EB family member 1, End-binding protein 1, End binding protein 1, APC-binding protein EB1

验证数据展示

  • WB analysis of HeLa using 17717-1-AP

    WB analysis of HeLa using 17717-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17717-1-AP (MAPRE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 17717-1-AP

    IHC staining of human kidney using 17717-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with MAPRE1/EB1 antibody included
  • IHC staining of human kidney using 17717-1-AP

    IHC staining of human kidney using 17717-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with MAPRE1/EB1 antibody included
  • WB analysis of HeLa using 17717-1-AP

    WB analysis of HeLa using 17717-1-AP

    WB result of EB1 antibody (17717-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EB1 transfected HeLa cells.

  • IP experiment of HeLa using 17717-1-AP

    IP experiment of HeLa using 17717-1-AP

    IP result of anti-EB1 (IP:17717-1-AP, 4ug; Detection:17717-1-AP 1:1000) with HeLa cells lysate 1200ug.

  • FC experiment of HeLa using 17717-1-AP

    FC experiment of HeLa using 17717-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human EB1 (17717-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human colon using 17717-1-AP

    IHC staining of human colon using 17717-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17717-1-AP (EB1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAPRE1/EB1 antibody included
  • IHC staining of human colon using 17717-1-AP

    IHC staining of human colon using 17717-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17717-1-AP (EB1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAPRE1/EB1 antibody included
  • IF Staining of HeLa using 17717-1-AP

    IF Staining of HeLa using 17717-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EB1 antibody (17717-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, A431 cells
Positive IP detected inHeLa cells, Caco-2 cells, mouse thymus tissue
Positive IHC detected inhuman kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human spleen tissue, human lung tissue, human normal colon, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MAPRE1/EB1 Ready-To-Use IHC Kit. MAPRE1/EB1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 4 publications below

产品信息

17717-1-AP targets EB1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen EB1 fusion protein Ag12092 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name microtubule-associated protein, RP/EB family, member 1
Synonyms MAPRE1, Microtubule-associated protein RP/EB family member 1, End-binding protein 1, End binding protein 1, APC-binding protein EB1
Calculated Molecular Weight 268 aa, 30 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC106068
Gene Symbol EB1
Gene ID (NCBI) 22919
RRIDAB_2141624
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15691
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EB1 antibody 17717-1-APDownload protocol
IHC protocol for EB1 antibody 17717-1-APDownload protocol
IF protocol for EB1 antibody 17717-1-APDownload protocol
IP protocol for EB1 antibody 17717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Nat Commun

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Authors - Hai-Qing Tu
View Article
humanIF

Elife

α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization.

Authors - Dandan Ma
View Article
mouseIF

Biol Open

MACF1 promotes preosteoblast migration by mediating focal adhesion turnover through EB1

Authors - Peihong Su
View Article
mouseIF

Biol Open

MACF1 promotes preosteoblast migration by mediating focal adhesion turnover through EB1.

Authors - Peihong Su
View Article
humanIF

Molecules

Pyrrolyldihydropyrazino[1,2-a]indoletrione Analogue Microtubule Inhibitor Induces Cell-Cycle Arrest and Apoptosis in Colorectal Cancer Cells

Authors - Dong-Lin Yang
View Article