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MARCH5 Polyclonal antibody

MARCH5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12213-1-AP

Print datasheet

Synonyms

MARCH V, MARCH5, Mitochondrial ubiquitin ligase, MITOL, RING finger protein 153, RNF153

验证数据展示

  • WB analysis of HeLa using 12213-1-AP

    WB analysis of HeLa using 12213-1-AP

    WB result of MARCH5 antibody (12213-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-MARCH5 transfected HeLa cells.

  • IHC staining of human skeletal muscle using 12213-1-AP

    IHC staining of human skeletal muscle using 12213-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12213-1-AP (MARCH5 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 12213-1-AP

    IHC staining of human skeletal muscle using 12213-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12213-1-AP (MARCH5 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, U-937 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12213-1-AP targets MARCH5 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen MARCH5 fusion protein Ag2806 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name membrane-associated ring finger (C3HC4) 5
Synonyms MARCH V, MARCH5, Mitochondrial ubiquitin ligase, MITOL, RING finger protein 153, RNF153
Calculated Molecular Weight 278 aa, 31 kDa
Observed Molecular Weight 31 kDa, 65-70 kDa
GenBank Accession NumberBC015480
Gene Symbol MARCH5
Gene ID (NCBI) 54708
RRIDAB_10638602
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX47
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MARCH5(E3 ubiquitin-protein ligase MARCH5) is a 31 kDa protein also named as RNF153. This protein plays a protective role against mitochondrial dysfunction caused by the mitochondrial accumulation of mSOD1 via the ubiquitin-proteasome pathway (PMID:19741096). And it may regulate MAP1B LC1 function in mitochondria by controlling mitochondrial aggregation via mitochondrial LC1 levels(PMID:22308378). MEARCH5 can exsit as a homodimer with MW 65-70 kDa.

实验方案

Product Specific Protocols
WB protocol for MARCH5 antibody 12213-1-APDownload protocol
IHC protocol for MARCH5 antibody 12213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Clin Transl Med

FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression.

Authors - Lei Miao
View Article