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  • KD/KO Validated

MARCH8 Polyclonal antibody

MARCH8 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 14119-1-AP

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Synonyms

c MIR, MARCH VIII, MARCH8, MIR, RING finger protein 178, RNF178



经过测试的应用

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14119-1-AP targets MARCH8 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, zebrafish
Immunogen MARCH8 fusion protein Ag5268 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name membrane-associated ring finger (C3HC4) 8
Synonyms c MIR, MARCH VIII, MARCH8, MIR, RING finger protein 178, RNF178
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 30-33 kDa
GenBank Accession NumberBC066988
Gene Symbol MARCH8
Gene ID (NCBI) 220972
RRIDAB_2140168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T0T0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MARCH8 (Membrane-associated RING finger protein 8) is also named as MIR, RNF178 and belongs to the RING zinc finger protein family. It functions as an E3 ubiquitin ligase for immune recognition-related molecules (e.g. major histocompatibility complex class I, B7-2, and ICAM-1). It plays a key role in controlling MHC class II surface expression by regulated ubiquitination of a lysine residue in the beta-chain (PMID:22761441).

实验方案

Product Specific Protocols
WB protocol for MARCH8 antibody 14119-1-APDownload protocol
IP protocol for MARCH8 antibody 14119-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

PMID: 28965816

Authors - Shouheng Jin
  • KO Validated
humanWB

Nat Commun

MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.

Authors - Xiaoman Liu

Autophagy

BST2 suppresses porcine epidemic diarrhea virus replication by targeting and degrading virus nucleocapsid protein with selective autophagy.

Authors - Ning Kong
  • KD Validated
humanWB

Autophagy

2AB protein of Senecavirus A antagonizes selective autophagy and type I interferon production by degrading LC3 and MARCHF8.

Authors - Dage Sun
  • KD Validated
humanWB

Proc Natl Acad Sci U S A

The E3 ubiquitin ligase MARCH8 negatively regulates IL-1β-induced NF-κB activation by targeting the IL1RAP coreceptor for ubiquitination and degradation.

Authors - Chen Rui R
humanWB

Cancers (Basel)

MARCH8 Suppresses Tumor Metastasis and Mediates Degradation of STAT3 and CD44 in Breast Cancer Cells.

Authors - Wenjing Chen