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验证数据展示

  • WB analysis using 14124-1-AP

    WB analysis using 14124-1-AP

    Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 14124-1-AP (MARCKSL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of skin cancer using 14124-1-AP

    IHC staining of skin cancer using 14124-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 14124-1-AP (MARCKSL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MARCKSL1 antibody included

MARCKSL1 Polyclonal antibody

MARCKSL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14124-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

MARCKS like protein 1, MARCKS like 1, Macrophage myristoylated alanine-rich C kinase substrate, Mac-MARCKS, MacMARCKS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected infetal human brain tissue, human brain tissue
Positive IHC detected inskin cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MARCKSL1 Ready-To-Use IHC Kit. MARCKSL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14124-1-AP targets MARCKSL1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 MARCKSL1 fusion protein Ag5274 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MARCKS-like 1
别名 MARCKS like protein 1, MARCKS like 1, Macrophage myristoylated alanine-rich C kinase substrate, Mac-MARCKS, MacMARCKS
计算分子量 20 kDa
观测分子量 48 kDa
GenBank蛋白编号BC066915
基因名称 MARCKSL1
Gene ID (NCBI) 65108
RRIDAB_2140454
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49006
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MARCKS-like protein 1 (MARCKSL1) is widely expressed in nervous tissue, it is also named MARCKS-like protein (MLP) or MARCKS-related protein (MRP) and belongs to the MARCKS family, which is a highly acidic myristoylated family of PKC substrates widely distributed in diverse cell types including macrophages. The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation. Genetic disruption of MARCKSL1 results in neural tube closure defects, events that depend on coordinated control of actin functions, cell shape, and cell migration. MARCKSL1 are thought to regulate the actin cytoskeleton and thereby participate in major cellular responses such as phagocytosis, secretion, motility, mitogenesis, and membrane trafficking.

实验方案

Product Specific Protocols
WB protocol for MARCKSL1 antibody 14124-1-APDownload protocol
IHC protocol for MARCKSL1 antibody 14124-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols