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验证数据展示

  • WB analysis using 14124-1-AP

    WB analysis using 14124-1-AP

    Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 14124-1-AP (MARCKSL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of skin cancer using 14124-1-AP

    IHC staining of skin cancer using 14124-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 14124-1-AP (MARCKSL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MARCKSL1 antibody included

MARCKSL1 Polyclonal antibody

MARCKSL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14124-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

MARCKS like protein 1, MARCKS like 1, Macrophage myristoylated alanine-rich C kinase substrate, Mac-MARCKS, MacMARCKS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected infetal human brain tissue, human brain tissue
Positive IHC detected inskin cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MARCKSL1 Ready-To-Use IHC Kit. MARCKSL1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14124-1-AP targets MARCKSL1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen MARCKSL1 fusion protein Ag5274 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MARCKS-like 1
Synonyms MARCKS like protein 1, MARCKS like 1, Macrophage myristoylated alanine-rich C kinase substrate, Mac-MARCKS, MacMARCKS
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC066915
Gene Symbol MARCKSL1
Gene ID (NCBI) 65108
RRIDAB_2140454
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49006
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MARCKS-like protein 1 (MARCKSL1) is widely expressed in nervous tissue, it is also named MARCKS-like protein (MLP) or MARCKS-related protein (MRP) and belongs to the MARCKS family, which is a highly acidic myristoylated family of PKC substrates widely distributed in diverse cell types including macrophages. The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation. Genetic disruption of MARCKSL1 results in neural tube closure defects, events that depend on coordinated control of actin functions, cell shape, and cell migration. MARCKSL1 are thought to regulate the actin cytoskeleton and thereby participate in major cellular responses such as phagocytosis, secretion, motility, mitogenesis, and membrane trafficking.

实验方案

Product Specific Protocols
WB protocol for MARCKSL1 antibody 14124-1-APDownload protocol
IHC protocol for MARCKSL1 antibody 14124-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols