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  • KD/KO Validated

MARK1 Polyclonal antibody

MARK1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21552-1-AP

Print datasheet

Synonyms

KIAA1477, MARK, MARK1



经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, SH-SY5Y cells, mouse kidney tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21552-1-AP is MARK1 Fusion Protein expressed in E. coli.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen MARK1 fusion protein Ag16225 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MAP/microtubule affinity-regulating kinase 1
Synonyms KIAA1477, MARK, MARK1
Calculated Molecular Weight 795 aa, 89 kDa
Observed Molecular Weight 85-89 kDa, 72 kDa
GenBank Accession NumberBC113869
Gene Symbol MARK1
Gene ID (NCBI) 4139
RRIDAB_10732726
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P0L2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MARK1, also named as MAP-microtubule affinity regulating kinase 1, which form a subfamily of the calcium/calmodulin-dependent protein kinase (CAMP) group of kinases(PMID: 22670221).It is involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. MARK1 also act as a regulator of the neuronal migration and Wnt signaling pathway.It has 3 isoforms which molecular weight is 89,84,72 kDa. This antibody may detect all of the isoforms.

实验方案

Product Specific Protocols
WB protocol for MARK1 antibody 21552-1-APDownload protocol
IHC protocol for MARK1 antibody 21552-1-APDownload protocol
IP protocol for MARK1 antibody 21552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

An AMPK-Independent Signaling Pathway Downstream of the LKB1 Tumor Suppressor Controls Snail1 and Metastatic Potential.

Authors - Jonathan M Goodwin
  • KD Validated
humanIP

Nat Commun

TNK1 is a ubiquitin-binding and 14-3-3-regulated kinase that can be targeted to block tumor growth.

Authors - Tsz-Yin Chan
  • KD Validated
mouseWB

Cell Rep

DIXDC1 Phosphorylation and Control of Dendritic Morphology Are Impaired by Rare Genetic Variants.

Authors - Vickie Kwan
humanWB

Cell Commun Signal

Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression.

Authors - Abiola Abdulrahman Ayanlaja
ratWB,IP

J Biol Chem

Postsynaptic density 95 (PSD-95) serine 561 phosphorylation regulates a conformational switch and bidirectional dendritic spine structural plasticity.

Authors - Qian Wu
ratWB

PLoS One

MARK/Par1 Kinase Is Activated Downstream of NMDA Receptors through a PKA-Dependent Mechanism.

Authors - Laura P Bernard