验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy MARS1 Ready-To-Use IHC Kit. MARS1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
14829-1-AP targets MARS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | MARS fusion protein Ag6619 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | methionyl-tRNA synthetase |
| 别名 | MARS1, EC:6.1.1.10, MTRNS, METRS, Methionyl-tRNA synthetase |
| 计算分子量 | 101 kDa |
| 观测分子量 | 101 kDa |
| GenBank蛋白编号 | BC002384 |
| 基因名称 | MARS |
| Gene ID (NCBI) | 4141 |
| RRID | AB_2140917 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P56192 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MARS antibody 14829-1-AP | Download protocol |
| IHC protocol for MARS antibody 14829-1-AP | Download protocol |
| IF protocol for MARS antibody 14829-1-AP | Download protocol |
| IP protocol for MARS antibody 14829-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging Cell N-homocysteinylation of DJ-1 promotes neurodegeneration in Parkinson's disease | ||
Proteomics Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes. | ||
J Clin Pathol High methionyl-tRNA synthetase expression predicts poor prognosis in patients with breast cancer.
| ||
Proc Natl Acad Sci U S A Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex
| ||
Aging Cell N-homocysteinylation of α-synuclein promotes its aggregation and neurotoxicity
|
