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验证数据展示

  • WB analysis of HeLa using 21837-1-AP

    WB analysis of HeLa using 21837-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21837-1-AP (MASP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 21837-1-AP

    IHC staining of human liver cancer using 21837-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21837-1-AP (MASP1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 21837-1-AP

    IHC staining of human liver cancer using 21837-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21837-1-AP (MASP1 Antibody) at dilution of 1:200 (under 40x lens).

  • Featured Product
  • KD/KO验证

MASP1 Polyclonal antibody

MASP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21837-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA and More (2)

别名

Complement factor MASP 3, CRARF, CRARF1, MASP, MASP 1, MASP1, PRSS5, RaRF, Serine protease 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21837-1-AP targets MASP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IP, IF, IHC
经测试反应性 human
文献引用反应性human, mouse
免疫原 MASP1 fusion protein Ag16432 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor)
别名 Complement factor MASP 3, CRARF, CRARF1, MASP, MASP 1, MASP1, PRSS5, RaRF, Serine protease 5
计算分子量 728 aa, 82 kDa
观测分子量 40-70 kDa
GenBank蛋白编号BC106945
基因名称 MASP1
Gene ID (NCBI) 5648
RRIDAB_2878924
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48740
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MASP1 antibody 21837-1-APDownload protocol
IHC protocol for MASP1 antibody 21837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Front Immunol

Thrombotic microangiopathy mediates poor prognosis among lupus nephritis via complement lectin and alternative pathway activation

Authors - Binshan Zhang
View Article
human,mouseWB,IHC,IP

Hepatology

Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis

Authors - Xianzhi Liu
  • KD Validated
View Article