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验证数据展示

  • WB analysis using 55252-1-AP

    WB analysis using 55252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55252-1-AP (MASP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 55252-1-AP

    IHC staining of human liver using 55252-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55252-1-AP (MASP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 55252-1-AP

    IHC staining of human liver using 55252-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55252-1-AP (MASP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MASP2 Polyclonal antibody

MASP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55252-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

MAP19, MASP 2, MASP2, sMAP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55252-1-AP targets MASP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mannan-binding lectin serine peptidase 2
别名 MAP19, MASP 2, MASP2, sMAP
计算分子量 76 kDa
观测分子量 68 kDa
GenBank蛋白编号NM_006610
基因名称 MASP2
Gene ID (NCBI) 10747
RRIDAB_3086437
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00187
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mannan-binding lectin serine protease 2 (MASP2) is a serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. MASP2 contains a 15-amino acid signal peptide, the 3 amino acids essential for the active center of a serine protease, and calcium-binding residues in the epidermal growth factor like domain.

实验方案

Product Specific Protocols
WB protocol for MASP2 antibody 55252-1-APDownload protocol
IHC protocol for MASP2 antibody 55252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols