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MASP2 Polyclonal antibody

MASP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55252-1-AP

Print datasheet

Synonyms

MAP19, MASP 2, MASP2, sMAP

验证数据展示

  • WB analysis using 55252-1-AP

    WB analysis using 55252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55252-1-AP (MASP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 55252-1-AP

    IHC staining of human liver using 55252-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55252-1-AP (MASP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 55252-1-AP

    IHC staining of human liver using 55252-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55252-1-AP (MASP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55252-1-AP targets MASP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mannan-binding lectin serine peptidase 2
Synonyms MAP19, MASP 2, MASP2, sMAP
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberNM_006610
Gene Symbol MASP2
Gene ID (NCBI) 10747
RRIDAB_3086437
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00187
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mannan-binding lectin serine protease 2 (MASP2) is a serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. MASP2 contains a 15-amino acid signal peptide, the 3 amino acids essential for the active center of a serine protease, and calcium-binding residues in the epidermal growth factor like domain.

实验方案

Product Specific Protocols
WB protocol for MASP2 antibody 55252-1-APDownload protocol
IHC protocol for MASP2 antibody 55252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols