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验证数据展示

  • WB analysis using 13305-1-AP

    WB analysis using 13305-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13305-1-AP (MAST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 13305-1-AP

    IP experiment of mouse brain using 13305-1-AP

    IP result of anti-MAST1 (IP:13305-1-AP, 4ug; Detection:13305-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • WB analysis of mouse brain using 13305-1-AP

    WB analysis of mouse brain using 13305-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13305-1-AP (MAST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

MAST1 Polyclonal antibody

MAST1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 13305-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA and More (2)

别名

SAST170, SAST, Microtubule-associated serine/threonine-protein kinase 1, MAST 1, KIAA0973

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13305-1-AP targets MAST1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 MAST1 fusion protein Ag4126 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 microtubule associated serine/threonine kinase 1
别名 SAST170, SAST, Microtubule-associated serine/threonine-protein kinase 1, MAST 1, KIAA0973
计算分子量 1570 aa, 171 kDa
观测分子量 171 kDa
GenBank蛋白编号BC027985
基因名称 MAST1
Gene ID (NCBI) 22983
RRIDAB_10639043
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2H9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAST1(microtubule associated serine/threonine kinase 1) is also named as KIAA0973, SAST(syntrophin-associated serine/threonine-protein kinase), SAST170 and belongs to the family of microtubule-associated serine/threonine kinases, which has a unique structural feature with a serine/threonine kinase domain and a postsynaptic density protein-95/discs large/zona occludens-1 domain(PMID:12614677). This protein plays a role in a broad range of neural functions and it appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins.

实验方案

Product Specific Protocols
WB protocol for MAST1 antibody 13305-1-APDownload protocol
IP protocol for MAST1 antibody 13305-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Neuron

Mutations in MAST1 Cause Mega-Corpus-Callosum Syndrome with Cerebellar Hypoplasia and Cortical Malformations.

Authors - Ratna Tripathy
  • KO Validated
View Article
humanWB,IHC,IF,IP

Theranostics

CRISPR/Cas9-based genome-wide screening for deubiquitinase subfamily identifies USP1 regulating MAST1-driven cisplatin-resistance in cancer cells

Authors - Apoorvi Tyagi
View Article