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验证数据展示

  • WB analysis of Jurkat using 19507-1-AP

    WB analysis of Jurkat using 19507-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 19507-1-AP

    IHC staining of human kidney using 19507-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19507-1-AP (MAST3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 19507-1-AP

    IHC staining of human kidney using 19507-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19507-1-AP (MAST3 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 19507-1-AP

    IF Staining of HepG2 using 19507-1-AP

    Immunofluorescent analysis of HepG2 cells, using MAST3 antibody 19507-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of Jurkat using 19507-1-AP

    IP experiment of Jurkat using 19507-1-AP

    IP result of anti-MAST3 (IP:19507-1-AP, 4ug; Detection:19507-1-AP 1:500) with Jurkat cells lysate 4000ug.

  • WB analysis using 19507-1-AP

    WB analysis using 19507-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

MAST3 Polyclonal antibody

MAST3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 19507-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

KIAA0561, MAST3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, human brain tissue, mouse lung tissue, mouse brain tissue, rat brain tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19507-1-AP targets MAST3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 microtubule associated serine/threonine kinase 3
别名 KIAA0561, MAST3
计算分子量 143 kDa
观测分子量 144 kDa
GenBank蛋白编号NM_015016
基因名称 MAST3
Gene ID (NCBI) 23031
RRIDAB_10642565
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60307
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAST3, also named as KIAA0561, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. MAST3 catalyze the reaction: ATP + a protein = ADP + a phosphoprotein.

实验方案

Product Specific Protocols
WB protocol for MAST3 antibody 19507-1-APDownload protocol
IHC protocol for MAST3 antibody 19507-1-APDownload protocol
IF protocol for MAST3 antibody 19507-1-APDownload protocol
IP protocol for MAST3 antibody 19507-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols