验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, HepG2 cells, HCT 116 cells, arabidopsis whole plant tissue, rat liver tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
12395-1-AP targets MAT1A/MAT2A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, arabidopsis samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat, arabidopsis |
| Cited Reactivity | human, mouse, rat |
| Immunogen | MAT1A/MAT2A fusion protein Ag3076 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | methionine adenosyltransferase I, alpha |
| Synonyms | AdoMet synthase 1, AMS1, MAT, MAT 1, MAT I/III, MAT1A, MATA1, SAMS, SAMS1 |
| Calculated Molecular Weight | 395 aa, 44 kDa |
| Observed Molecular Weight | 44-55 kDa |
| GenBank Accession Number | BC018359 |
| Gene Symbol | MAT1A |
| Gene ID (NCBI) | 4143 |
| RRID | AB_2266186 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q00266 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Methionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A. This antibody was raised against the full length of human MAT1A and can recognize both of MAT1A and MAT2A. Western blot analysis using this antibody detected a single band around 44-50 kDa in liver.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAT1A/MAT2A antibody 12395-1-AP | Download protocol |
| IHC protocol for MAT1A/MAT2A antibody 12395-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Pathol Forced expression of methionine adenosyltransferase 1A in human hepatoma cells suppresses in vivo tumorigenicity in mice. | ||
Toxicol Sci Metabolomic Analysis Reveals Metabolic Changes Caused By Bisphenol A in Rats. | ||
Proteomics Early changes in the liver-soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet. | ||
J Surg Oncol Histological expression of methionine adenosyl transferase (MAT) 2A as a post-surgical prognostic surrogate in patients with hepatocellular carcinoma. |
