验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, mouse thymus tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
10082-2-AP targets MATK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | MATK fusion protein Ag0128 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | megakaryocyte-associated tyrosine kinase |
| 别名 | Tyrosine-protein kinase CTK, Megakaryocyte-associated tyrosine-protein kinase, HYL, Hematopoietic consensus tyrosine-lacking kinase, EC:2.7.10.2 |
| 计算分子量 | 56 kDa |
| 观测分子量 | 56 kDa |
| GenBank蛋白编号 | BC000114 |
| 基因名称 | MATK |
| Gene ID (NCBI) | 4145 |
| RRID | AB_2141386 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P42679 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MATK(Megakaryocyte-associated tyrosine-protein kinase) is also named as CTK, HYL and belongs to the protein kinase superfamily. This protein can can inhibit the kinase activity of certain Src kinase family members by phosphorylating the C-terminal tyrosine and by a non-catalytic mechanism. It may play an inhibitory role in the control of T-cell proliferation. MATK has 3 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MATK antibody 10082-2-AP | Download protocol |
| IHC protocol for MATK antibody 10082-2-AP | Download protocol |
| IF protocol for MATK antibody 10082-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother Anti-osteoporotic effects of alisol C 23-acetate via osteoclastogenesis inhibition. | ||
