MATK Polyclonal antibody

MATK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10082-2-AP

Print datasheet

Synonyms

Tyrosine-protein kinase CTK, Megakaryocyte-associated tyrosine-protein kinase, HYL, Hematopoietic consensus tyrosine-lacking kinase, EC:2.7.10.2



经过测试的应用

Positive WB detected inK-562 cells, mouse thymus tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

10082-2-AP targets MATK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen MATK fusion protein Ag0128 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name megakaryocyte-associated tyrosine kinase
Synonyms Tyrosine-protein kinase CTK, Megakaryocyte-associated tyrosine-protein kinase, HYL, Hematopoietic consensus tyrosine-lacking kinase, EC:2.7.10.2
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC000114
Gene Symbol MATK
Gene ID (NCBI) 4145
RRIDAB_2141386
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42679
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MATK(Megakaryocyte-associated tyrosine-protein kinase) is also named as CTK, HYL and belongs to the protein kinase superfamily. This protein can can inhibit the kinase activity of certain Src kinase family members by phosphorylating the C-terminal tyrosine and by a non-catalytic mechanism. It may play an inhibitory role in the control of T-cell proliferation. MATK has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for MATK antibody 10082-2-APDownload protocol
IHC protocol for MATK antibody 10082-2-APDownload protocol
IF protocol for MATK antibody 10082-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Biomed Pharmacother

Anti-osteoporotic effects of alisol C 23-acetate via osteoclastogenesis inhibition.

Authors - Xiaokang Jia
humanWB

Biophys Rep

Detection of ribophagy in yeast and mammals

Authors - Miao Ye