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验证数据展示

  • WB analysis using 28566-1-AP

    WB analysis using 28566-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28566-1-AP (MATN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 28566-1-AP

    IF Staining of HeLa using 28566-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MATN1 antibody (28566-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

MATN1 Polyclonal antibody

MATN1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28566-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

pig, human

应用

WB, IF/ICC, ELISA

别名

Cartilage matrix protein, CMP, CRTM, MATN1, Matrilin 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig cartilage tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28566-1-AP targets MATN1 in WB, IF/ICC, ELISA applications and shows reactivity with pig, human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 pig, human
免疫原 MATN1 fusion protein Ag29386 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrilin 1, cartilage matrix protein
别名 Cartilage matrix protein, CMP, CRTM, MATN1, Matrilin 1
计算分子量 54 kDa
观测分子量54 kDa
GenBank蛋白编号NM_002379
基因名称 MATN1
Gene ID (NCBI) 4146
RRIDAB_3669662
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP21941
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MATN1 (matrilin 1), also known as CMP. It is expected to be located in extracellular space, the protein is mainly expressed in cartilage tissue and retina tissue. Also, the protein is a member of von Willebrand factor A domain containing protein family. This family of proteins are thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. Mutations of this gene have been associated with variety of inherited chondrodysplasias. It is reproted that COMP and matrilin-1 elute together in the gel filtration of cartilage extracts and can be co-immunoprecipitated with the presence of calcium (PMID: 15075323). The calculated molecular weight of MATN1 is 54 kDa.

实验方案

Product Specific Protocols
WB protocol for MATN1 antibody 28566-1-APDownload protocol
IF protocol for MATN1 antibody 28566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols