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MATN3 Polyclonal antibody

MATN3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25500-1-AP

Print datasheet

Synonyms

Matrilin-3, matrilin 3, HOA, EDM5

验证数据展示

  • WB analysis using 25500-1-AP

    WB analysis using 25500-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25500-1-AP (MATN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 25500-1-AP

    IHC staining of human stomach cancer using 25500-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25500-1-AP (MATN3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman milk
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25500-1-AP targets MATN3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen MATN3 fusion protein Ag21932 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name matrilin 3
Synonyms Matrilin-3, matrilin 3, HOA, EDM5
Calculated Molecular Weight 486 aa, 53 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC139907
Gene Symbol MATN3
Gene ID (NCBI) 4148
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15232
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Matrilin-3 is a protein that in humans is encoded by the MATN3 gene. It is linked to the development of many types of cartilage, and part of the Matrilin family, which includes Matrilin-1, Matrilin-2, Matrilin-3, and Matrilin-4, a family of filamentous-forming adapter oligomeric extracellular proteins that are linked to the formation of cartilage and bone, as well as maintaining homeostasis after development. It is considered an extracellular matrix protein that functions as an adapter protein where the Matrilin-3 subunit can form both homo-tetramers and hetero-oligomers with subunits from Matrilin-1 which is the cartilage matrix protein. This restricted tissue has been strongly expressed in growing skeletal tissue as well as cartilage and bone.( PMID:26499313, 27104523, 11950247)

实验方案

Product Specific Protocols
WB protocol for MATN3 antibody 25500-1-APDownload protocol
IHC protocol for MATN3 antibody 25500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols