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验证数据展示

  • WB analysis using 68280-1-Ig

    WB analysis using 68280-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68280-1-Ig (MATR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis using 68280-1-Ig

    WB analysis using 68280-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68280-1-Ig (MATR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 68280-1-Ig

    IF Staining of HEK-293 using 68280-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MATR3 antibody (68280-1-Ig, Clone: 3B12C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

MATR3 Monoclonal antibody

MATR3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68280-1-Ig

产品说明书

CloneNo. 3B12C7

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Matrin-3, matrin 3, KIAA0723

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, rat brain tissue, A431 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, mouse brain tissue
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy MATR3 Ready-To-Use IHC Kit. MATR3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68280-1-Ig targets MATR3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MATR3 fusion protein Ag31502 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 matrin 3
别名 Matrin-3, matrin 3, KIAA0723
计算分子量 847 aa, 95 kDa
观测分子量120 kDa
GenBank蛋白编号BC015031
基因名称 MATR3
Gene ID (NCBI) 9782
RRIDAB_2935361
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP43243
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MATR3, known as KIAA0723, is an 847 amino acid protein, which may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. MATR3 was originally identified as a nuclear matrix protein containing both DNA and RNA binding domains (PMID: 2033075) with a wide variety of functions ranging from DNA damage response to transcriptional and post-transcriptional regulation of cellular and viral RNAs(PMID: 21771346). the calculated molecular weight of MATR3 is 94 kDa, but we detect a 125 kDa protein as a result of PMID: 28977530.

实验方案

Product Specific Protocols
WB protocol for MATR3 antibody 68280-1-IgDownload protocol
IF protocol for MATR3 antibody 68280-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols