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MAVS; VISA Recombinant antibody, PBS Only

MAVS; VISA Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

7F14

Cat no : 81910-1-PBS

Print datasheet

Synonyms

MAVS, KIAA1271, IPS-1, IPS1, IPS 1

验证数据展示

  • WB analysis using 81910-1-RR (same clone as 81910-1-PBS)

    WB analysis using 81910-1-RR (same clone as 81910-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81910-1-RR (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 81910-1-RR (same clone as 81910-1-PBS)

    IP experiment of HEK-293 using 81910-1-RR (same clone as 81910-1-PBS)

    IP result of anti-MAVS; VISA (IP:81910-1-RR, 4ug; Detection:81910-1-RR 1:4000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81910-1-RR (MAVS; VISA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81910-1-RR (MAVS; VISA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAVS; VISA antibody (81910-1-RR, Clone: 7F14 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAVS; VISA antibody (81910-1-RR, Clone: 7F14 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81910-1-PBS

    Affinity and Kinetic Characterization of 81910-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 81910-1-RR against Human MAVS; VISA were performed. The affinity constant is below 1 pM.

产品信息

81910-1-PBS targets MAVS; VISA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen MAVS; VISA fusion protein Ag5655 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitochondrial antiviral signaling protein
Synonyms MAVS, KIAA1271, IPS-1, IPS1, IPS 1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC044952
Gene Symbol MAVS
Gene ID (NCBI) 57506
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7Z434
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN-β promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.