验证数据展示
经过测试的应用
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | U2OS cells, HeLa cells |
推荐稀释比
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82852-3-RR targets MAZ in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | MAZ fusion protein Ag15200 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | MYC-associated zinc finger protein (purine-binding transcription factor) |
Synonyms | Pur-1, PUR1, Pur 1, Myc-associated zinc finger protein, MAZI |
Calculated Molecular Weight | 477 aa, 49 kDa |
Observed Molecular Weight | 49-51 kDa |
GenBank Accession Number | BC041629 |
Gene Symbol | MAZ |
Gene ID (NCBI) | 4150 |
RRID | AB_3086554 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P56270 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Myc-associated zinc-finger protein (MAZ) is a transcription factor with dual roles in transcription initiation and termination. MAZ can activate the expression of a variety of genes such as the c-Myc, insulin, VEGF, and RAS gene family and the serotonin 1A receptor. MAZ plays a critical role in the progression of various cancers including breast, prostate, liver, and so on. Deregulation of MAZ expression is associated with the progression of pancreatic ductal adenocarcinoma (PDAC)(PMID: 29414775).
实验方案
Product Specific Protocols | |
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IHC protocol for MAZ antibody 82852-3-RR | Download protocol |
IF protocol for MAZ antibody 82852-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |