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  • Featured Product
  • KD/KO Validated

MBD3 Polyclonal antibody, PBS Only

MBD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14258-1-PBS

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Synonyms

验证数据展示

  • WB analysis of Y79 using 14258-1-AP (same clone as 14258-1-PBS)

    WB analysis of Y79 using 14258-1-AP (same clone as 14258-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 14258-1-AP (same clone as 14258-1-PBS)

    IHC staining of human endometrial cancer using 14258-1-AP (same clone as 14258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14258-1-AP (MBD3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 14258-1-AP (same clone as 14258-1-PBS)

    IF Staining of HepG2 using 14258-1-AP (same clone as 14258-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using MBD3 antibody 14258-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 14258-1-AP (same clone as 14258-1-PBS)

    IP experiment of mouse testis using 14258-1-AP (same clone as 14258-1-PBS)

    IP result of anti-MBD3 (IP:14258-1-AP, 4ug; Detection:14258-1-AP 1:800) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

产品信息

14258-1-PBS targets MBD3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MBD3 fusion protein Ag5531 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name methyl-CpG binding domain protein 3
Synonyms
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC043619
Gene Symbol MBD3
Gene ID (NCBI) 53615
RRIDAB_2139745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95983
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MBD3 does not bind DNA by itself. It recruits histone deacetylases and DNA methyltransferases. MBD3 acts as transcriptional repressor and plays a role in gene silencing.(PMID:9774669) .