验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human testis tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy MBIP Ready-To-Use IHC Kit. MBIP primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
产品信息
10685-1-AP targets MBIP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | MBIP fusion protein Ag1061 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | MAP3K12 binding inhibitory protein 1 |
| Synonyms | MUK-binding inhibitory protein, MUK binding inhibitory protein, MAPK upstream kinase-binding inhibitory protein, MAP3K12-binding inhibitory protein 1, BM-015 |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC005197 |
| Gene Symbol | MBIP |
| Gene ID (NCBI) | 51562 |
| RRID | AB_2139889 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NS73 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MBIP antibody 10685-1-AP | Download protocol |
| IHC protocol for MBIP antibody 10685-1-AP | Download protocol |
| IF protocol for MBIP antibody 10685-1-AP | Download protocol |
| IP protocol for MBIP antibody 10685-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J Distinct roles of GCN5/PCAF-mediated H3K9ac and CBP/p300-mediated H3K18/27ac in nuclear receptor transactivation. | ||
Mol Cell Biol The double-histone-acetyltransferase complex ATAC is essential for mammalian development. | ||
J Biol Chem Endoplasmic reticulum stress-responsive transcription factor ATF6α directs recruitment of the Mediator of RNA polymerase II transcription and multiple histone acetyltransferase complexes. | ||
J Biol Chem Human ATAC Is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein. |
