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验证数据展示

  • WB analysis of mouse skeletal muscle using 24610-1-AP

    WB analysis of mouse skeletal muscle using 24610-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24610-1-AP (MBNL3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IHC staining of human skeletal muscle using 24610-1-AP

    IHC staining of human skeletal muscle using 24610-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24610-1-AP (MBNL3 Antibody) at dilution of 1:50 (under 40x lens).

MBNL3 Polyclonal antibody

MBNL3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24610-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

CHCR, Cys3His CCG1 required protein, MBLX, MBLX39, MBNL3, MBXL, Muscleblind like protein 3, Protein HCHCR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

24610-1-AP targets MBNL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 MBNL3 fusion protein Ag20113 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 muscleblind-like 3 (Drosophila)
别名 CHCR, Cys3His CCG1 required protein, MBLX, MBLX39, MBNL3, MBXL, Muscleblind like protein 3, Protein HCHCR
计算分子量 354 aa, 39 kDa
观测分子量 38 kDa
GenBank蛋白编号BC042090
基因名称 MBNL3
Gene ID (NCBI) 55796
RRIDAB_2879637
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NUK0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MBNL3, also named as Muscleblind-like protein 3 or Cys3His CCG1-required protein, is a 354 amino acid protein, which contains four C3H1-type zinc fingers and belongs to the muscleblind family. MBNL3 localizes in the nucleus and is highly expressed in the placenta. MBNL3 mediates pre-mRNA alternative splicing regulation and plays a role in myotonic dystrophy pathophysiology. MBNL3 could inhibit terminal muscle differentiation, acting at approximately the time of myogenin induction.

实验方案

Product Specific Protocols
WB protocol for MBNL3 antibody 24610-1-APDownload protocol
IHC protocol for MBNL3 antibody 24610-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Cycle

TRIM71 binds to IMP1 and is capable of positive and negative regulation of target RNAs.

Authors - Daniel J Foster
View Article