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验证数据展示

  • WB analysis using 32120-1-AP

    WB analysis using 32120-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32120-1-AP (MBOAT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

MBOAT4 Polyclonal antibody

MBOAT4 Polyclonal Antibody for WB, ELISA
Cat No. 32120-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

OACT4, Membrane-bound O-acyltransferase domain-containing protein 4, GOAT, Ghrelin O-acyltransferase, FKSG89

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32120-1-AP targets MBOAT4 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 MBOAT4 fusion protein Ag37237 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 membrane bound O-acyltransferase domain containing 4
别名 OACT4, Membrane-bound O-acyltransferase domain-containing protein 4, GOAT, Ghrelin O-acyltransferase, FKSG89
计算分子量50 kDa
观测分子量50 kDa
GenBank蛋白编号NM_001100916
基因名称 MBOAT4
Gene ID (NCBI) 619373
RRIDAB_3670198
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ96T53
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MBOAT4, also known as ghrelin O-acyltransferase (GOAT), is an endoplasmic reticulum enzyme that catalyzes the octanoylation of the peptide hormone ghrelin, which is essential for the binding of ghrelin to its receptor and the subsequent regulation of food intake, growth hormone secretion, and inhibition of insulin secretion (PMID: 34946685). MBOAT4 is considered a potential therapeutic target for treating disorders related to ghrelin signaling (PMID: 32564644).

实验方案

Product Specific Protocols
WB protocol for MBOAT4 antibody 32120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols