验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26646-1-AP targets MBOAT7 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | MBOAT7 fusion protein Ag24490 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | membrane bound O-acyltransferase domain containing 7 |
| 别名 | hMBOA 7, h mboa 7, EC:2.3.1.-, Bladder and breast carcinoma-overexpressed gene 1 protein, BB1 |
| 计算分子量 | 472 aa, 53 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC006309 |
| 基因名称 | MBOAT7 |
| Gene ID (NCBI) | 79143 |
| RRID | AB_3085890 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96N66 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MBOAT7 (membrane-bound O-acyltransferase domain containing 7), also known as LPIAT, LENG4, or BB1, belongs to the MBOAT superfamily. MBOAT7 acylates lyso-phosphatidylinositol (lyso-PI) with arachidonyl-CoA and is involved in phosphatidylinositol acyl-chain remodeling in the Lands cycle (PMID: 30959108; PMID: 35509994). MBOAT7 deficiency in humans leads to developmental disorders of the central nervous system, with intellectual disability, epilepsy, and autism spectrum disorder (PMID: 37316513). The calculated molecular weight of MBOAT7 is 53 kDa. This antibody can recognize the 45 kDa isoform of MBOAT7.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MBOAT7 antibody 26646-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |