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MBOAT7 Recombinant antibody

MBOAT7 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240505E8

Cat no : 83546-3-RR

Print datasheet

Synonyms

h mboa 7, EC:2.3.1.-, Bladder and breast carcinoma-overexpressed gene 1 protein, BB1, 240505E8

验证数据展示

  • IHC staining of mouse testis using 83546-3-RR

    IHC staining of mouse testis using 83546-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83546-3-RR (MBOAT7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 83546-3-RR

    IHC staining of mouse testis using 83546-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83546-3-RR (MBOAT7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HuH-7 using 83546-3-RR

    IF Staining of HuH-7 using 83546-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using MBOAT7 antibody (83546-3-RR, Clone: 240505E8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A549 using 83546-3-RR

    FC experiment of A549 using 83546-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug MBOAT7 Recombinant antibody (83546-3-RR, Clone:240505E8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83546-3-RR

    Affinity and Kinetic Characterization of 83546-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83546-3-RR against Human MBOAT7 were performed. The affinity constant is 0.573 nM.

经过测试的应用

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHuH-7 cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83546-3-RR targets MBOAT7 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen MBOAT7 fusion protein Ag24466 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name membrane bound O-acyltransferase domain containing 7
Synonyms h mboa 7, EC:2.3.1.-, Bladder and breast carcinoma-overexpressed gene 1 protein, BB1, 240505E8
Calculated Molecular Weight 472 aa, 53 kDa
GenBank Accession NumberBC006309
Gene Symbol MBOAT7
Gene ID (NCBI) 79143
RRIDAB_3671165
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96N66
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MBOAT7 (membrane-bound O-acyltransferase domain containing 7), also known as LPIAT, LENG4, or BB1, belongs to the MBOAT superfamily. MBOAT7 acylates lyso-phosphatidylinositol (lyso-PI) with arachidonyl-CoA and is involved in phosphatidylinositol acyl-chain remodeling in the Lands cycle (PMID: 30959108; PMID: 35509994). MBOAT7 deficiency in humans leads to developmental disorders of the central nervous system, with intellectual disability, epilepsy, and autism spectrum disorder (PMID: 37316513). The calculated molecular weight of MBOAT7 is 53 kDa. This antibody can recognize the 45 kDa isoform of MBOAT7.

实验方案

Product Specific Protocols
IHC protocol for MBOAT7 antibody 83546-3-RRDownload protocol
IF protocol for MBOAT7 antibody 83546-3-RRDownload protocol
FC protocol for MBOAT7 antibody 83546-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols