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MCHR1 Polyclonal antibody

MCHR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11656-2-AP

Print datasheet

Synonyms

MCH receptor 1, MCH R1, MCH 1R, G-protein coupled receptor 24, GPR24

验证数据展示

  • IHC staining of mouse heart using 11656-2-AP

    IHC staining of mouse heart using 11656-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11656-2-AP (MCHR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of SH-SY5Y using 11656-2-AP

    WB analysis of SH-SY5Y using 11656-2-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11656-2-AP (MCHR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse heart tissue, human brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11656-2-AP targets MCHR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MCHR1 fusion protein Ag2224 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name melanin-concentrating hormone receptor 1
Synonyms MCH receptor 1, MCH R1, MCH 1R, G-protein coupled receptor 24, GPR24
Calculated Molecular Weight 422 aa, 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC021146
Gene Symbol MCHR1
Gene ID (NCBI) 2847
RRIDAB_2143940
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99705
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MCHR1 antibody 11656-2-APDownload protocol
IHC protocol for MCHR1 antibody 11656-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols