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验证数据展示

  • IHC staining of mouse heart using 11656-2-AP

    IHC staining of mouse heart using 11656-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11656-2-AP (MCHR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of SH-SY5Y using 11656-2-AP

    WB analysis of SH-SY5Y using 11656-2-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11656-2-AP (MCHR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MCHR1 Polyclonal antibody

MCHR1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11656-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MCH receptor 1, MCH R1, MCH 1R, G-protein coupled receptor 24, GPR24

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse heart tissue, human brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11656-2-AP targets MCHR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MCHR1 fusion protein Ag2224 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 melanin-concentrating hormone receptor 1
别名 MCH receptor 1, MCH R1, MCH 1R, G-protein coupled receptor 24, GPR24
计算分子量 422 aa, 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC021146
基因名称 MCHR1
Gene ID (NCBI) 2847
RRIDAB_2143940
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99705
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MCHR1 antibody 11656-2-APDownload protocol
IHC protocol for MCHR1 antibody 11656-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols