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验证数据展示

  • WB analysis using 67989-1-Ig

    WB analysis using 67989-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67989-1-Ig (MCM6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • WB analysis of HeLa using 67989-1-Ig

    WB analysis of HeLa using 67989-1-Ig

    WB result of MCM6 antibody (67989-1-Ig; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM6 transfected HeLa cells.

  • IHC staining of human cervical cancer using 67989-1-Ig

    IHC staining of human cervical cancer using 67989-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67989-1-Ig (MCM6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MCM6 antibody included
  • IF Staining of HepG2 using 67989-1-Ig

    IF Staining of HepG2 using 67989-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using MCM6 antibody (67989-1-Ig, Clone: 2E12H1 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

  • Featured Product
  • KD/KO验证

MCM6 Monoclonal antibody

MCM6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67989-1-Ig

产品说明书

CloneNo. 2E12H1

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat, Mouse

应用

WB, IHC, IF/ICC, ELISA

别名

MCG40308, MCM6, Mis5, P105MCM

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HEK-293 cells, COLO 320 cells, HepG2 cells, MCF-7 cells, Jurkat cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MCM6 Ready-To-Use IHC Kit. MCM6 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67989-1-Ig targets MCM6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Rat, Mouse
免疫原 MCM6 fusion protein Ag4354 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 minichromosome maintenance complex component 6
别名 MCG40308, MCM6, Mis5, P105MCM
计算分子量 821 aa, 93 kDa
观测分子量105 kDa
GenBank蛋白编号BC032374
基因名称 MCM6
Gene ID (NCBI) 4175
RRIDAB_2918738
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14566
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027]. The calculated molecular weight of MCM6 is 92 kDa, but the modified MCM6 is about 105 kDa.

实验方案

Product Specific Protocols
WB protocol for MCM6 antibody 67989-1-IgDownload protocol
IHC protocol for MCM6 antibody 67989-1-IgDownload protocol
IF protocol for MCM6 antibody 67989-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols