验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, NIH/3T3 cells |
| Positive IP detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16451-1-AP targets MCM8 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MCM8 fusion protein Ag8696 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | minichromosome maintenance complex component 8 |
| 别名 | C20orf154, MCM8, Minichromosome maintenance 8, REC |
| 计算分子量 | 880 aa, 98 kDa |
| 观测分子量 | 90-95 kDa |
| GenBank蛋白编号 | BC008830 |
| 基因名称 | MCM8 |
| Gene ID (NCBI) | 84515 |
| RRID | AB_2142833 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UJA3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MCM8, also named as Minichromosome maintenance 8, is a 840 amino acid protein, which belongs to the MCM family. MCM8 is expressed highly in placenta, lung and pancreas and various tumors with highest levels in colon and lung cancers. MCM8 is a component of the MCM8-MCM9 complex, which involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. MCM8 exists some isoforms with MV 94 kDa and 70 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MCM8 antibody 16451-1-AP | Download protocol |
| IP protocol for MCM8 antibody 16451-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain. | ||
Nat Commun MCM8IP activates the MCM8-9 helicase to promote DNA synthesis and homologous recombination upon DNA damage. | ||
Cell Death Dis Aberrantly expressed HORMAD1 disrupts nuclear localization of MCM8-MCM9 complex and compromises DNA mismatch repair in cancer cells. | ||
Nat Commun A multi-functional role for the MCM8/9 helicase complex in maintaining fork integrity during replication stress |