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MCOLN1 Polyclonal antibody

MCOLN1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15291-1-AP

Print datasheet

Synonyms

MSTP080, MST080, MLIV, ML4, ML1

验证数据展示

  • WB analysis using 15291-1-AP

    WB analysis using 15291-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15291-1-AP (MCOLN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 15291-1-AP

    IHC staining of mouse brain using 15291-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15291-1-AP (MCOLN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 15291-1-AP

    IF Staining of U2OS using 15291-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using MCOLN1 antibody (15291-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman placenta tissue, mouse kidney tissue, rat kidney tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15291-1-AP targets MCOLN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen MCOLN1 fusion protein Ag7493 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mucolipin 1
Synonyms MSTP080, MST080, MLIV, ML4, ML1
Calculated Molecular Weight 65 kDa
Observed Molecular Weight60-65 kDa
GenBank Accession NumberBC005149
Gene Symbol MCOLN1
Gene ID (NCBI) 57192
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZU1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MCOLN1, also named MLIV, ML4, MG-2, and Mucolipidin, belongs to the transient receptor (TC 1.A.4) family, Polycystin subfamily, and MCOLN1 sub-subfamily. It is playing a role in the endocytic pathway and in the control of membrane trafficking of proteins and lipids. MCOLN1 may play a major role in Ca2+ transport regulating lysosomal exocytosis. It is an autosomal recessive lysosomal storage disorder characterized by severe psychomotor retardation and ophthalmologic abnormalities, including corneal opacity, retinal degeneration, and strabismus.

实验方案

Product Specific Protocols
WB protocol for MCOLN1 antibody 15291-1-APDownload protocol
IHC protocol for MCOLN1 antibody 15291-1-APDownload protocol
IF protocol for MCOLN1 antibody 15291-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Autophagy

Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

Authors - Kui Wang
View Article
mouse,ratWB

Biochim Biophys Acta Mol Basis Dis

TRPML1 ameliorates seizures-related neuronal injury by regulating autophagy and lysosomal biogenesis via Ca2+/TFEB signaling pathway

Authors - Tingting Peng
View Article