验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, HeLa cells, SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13879-1-AP targets MCOLN3 in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MCOLN3 fusion protein Ag4962 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mucolipin 3 |
| 别名 | Mucolipin 3, TRPML3, TRP ML3, Transient receptor potential channel mucolipin 3 |
| 计算分子量 | 64 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC060765 |
| 基因名称 | MCOLN3 |
| Gene ID (NCBI) | 55283 |
| RRID | AB_2142973 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TDD5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MCOLN3 antibody 13879-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Traffic The Ca(2+) channel TRPML3 regulates membrane trafficking and autophagy.
| ||
Cell Mol Gastroenterol Hepatol FGL2-MCOLN3-Autophagy Axis-Triggered Neutrophil Extracellular Traps Exacerbate Liver Injury in Fulminant Viral Hepatitis | ||
Phytomedicine Baicalin induces cell death of non-small cell lung cancer cells via MCOLN3-mediated lysosomal dysfunction and autophagy blockage
|