验证数据展示
经过测试的应用
| Positive WB detected in | PMA, LPS and Brefeldin A treated THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
29547-1-AP targets MCP-1/CCL2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 免疫原 | fusion protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) ligand 2 |
| 别名 | CCL2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2 |
| 计算分子量 | 99 aa, 11 kDa |
| 观测分子量 | 11 kDa |
| GenBank蛋白编号 | BC009716 |
| 基因名称 | MCP-1/CCL2 |
| Gene ID (NCBI) | 6347 |
| RRID | AB_2918323 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P13500 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MCP-1, ( also named as CCL2) is a member of the chemokine family. MCP-1 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. MCP-1 has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. Elevated expression of MCP-1 is associated with severe acute respiratory syndrome coronavirus 2 infection.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MCP-1/CCL2 antibody 29547-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |