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MCTS1 Polyclonal antibody

MCTS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14984-1-AP

Print datasheet

Synonyms

Multiple copies T-cell malignancies, MCT-1, MCT1, Malignant T-cell-amplified sequence 1

验证数据展示

  • WB analysis using 14984-1-AP

    WB analysis using 14984-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14984-1-AP (MCTS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse bladder using 14984-1-AP

    WB analysis of mouse bladder using 14984-1-AP

    mouse bladder tissue were subjected to SDS PAGE followed by western blot with 14984-1-AP (MCTS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovarian cancer using 14984-1-AP

    IHC staining of human ovarian cancer using 14984-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 14984-1-AP (MCTS1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, mouse bladder tissue, K-562 cells, MOLT-4 cells, Raji cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14984-1-AP targets MCTS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen MCTS1 fusion protein Ag6968 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name malignant T cell amplified sequence 1
Synonyms Multiple copies T-cell malignancies, MCT-1, MCT1, Malignant T-cell-amplified sequence 1
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 18-21 kDa
GenBank Accession NumberBC001013
Gene Symbol MCTS1
Gene ID (NCBI) 28985
RRIDAB_2878096
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULC4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MCTS1 is an anti-oncogene that plays a role in cell cycle regulation. It can reduce cell doubling time and anchor-dependent growth. The G1 transition time and the duration of the G1 / S transition are shortened. It can also promote the release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits (PMID: 10440924).

实验方案

Product Specific Protocols
WB protocol for MCTS1 antibody 14984-1-APDownload protocol
IHC protocol for MCTS1 antibody 14984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion.

Authors - Baiwen Chen
View Article