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MDC1 Polyclonal antibody

MDC1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22272-1-AP

Print datasheet

Synonyms

NFBD1, NFBD 1, MDC 1

验证数据展示

  • WB analysis using 22272-1-AP

    WB analysis using 22272-1-AP

    Total HEK-293 cells and HEK-293 nuclear extract lysates were subjected to SDS PAGE followed by western blot with 22272-1-AP (MDC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22272-1-AP targets MDC1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mediator of DNA damage checkpoint 1
Synonyms NFBD1, NFBD 1, MDC 1
Calculated Molecular Weight 227 kDa
Observed Molecular Weight~260 kDa
GenBank Accession NumberNM_014641
Gene Symbol MDC1
Gene ID (NCBI) 9656
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14676
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.

实验方案

Product Specific Protocols
WB protocol for MDC1 antibody 22272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols