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  • Featured Product
  • KD/KO Validated

MDH1 Monoclonal antibody, PBS Only

MDH1 Monoclonal Antibody for WB,Indirect ELISA,IF,FC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB,Indirect ELISA,IF,FC

Conjugate

Unconjugated

CloneNo.

1F9A2

Cat No : 66505-1-PBS

Print datasheet

Synonyms

Cytosolic malate dehydrogenase, MDH s, MDH1, MDHA, MOR2

验证数据展示

  • WB analysis using 66505-1-Ig (same clone as 66505-1-PBS)

    WB analysis using 66505-1-Ig (same clone as 66505-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66505-1-Ig (MDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66505-1-Ig (same clone as 66505-1-PBS)

    WB analysis of HEK-293 using 66505-1-Ig (same clone as 66505-1-PBS)

    WB result of MDH1 antibody (66505-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDH1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66505-1-Ig (same clone as 66505-1-PBS)

    IF Staining of HepG2 using 66505-1-Ig (same clone as 66505-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66505-1-Ig (MDH1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 66505-1-Ig (same clone as 66505-1-PBS)

    FC experiment of HEK-293 using 66505-1-Ig (same clone as 66505-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human MDH1 (66505-1-Ig, Clone:1F9A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

产品信息

66505-1-PBS targets MDH1 in WB, IF, FC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB,Indirect ELISA,IF,FC Application Description
Tested Reactivity Human, mouse
Immunogen MDH1 fusion protein Ag8744 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name malate dehydrogenase 1, NAD (soluble)
Synonyms Cytosolic malate dehydrogenase, MDH s, MDH1, MDHA, MOR2
Calculated Molecular Weight 334 aa, 36 kDa
Observed Molecular Weight 36-37 kDa
GenBank Accession NumberBC001484
Gene Symbol MDH1
Gene ID (NCBI) 4190
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP40925
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme. (PMID:10693743)