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验证数据展示

  • WB analysis using 84576-4-RR (same clone as 84576-4-PBS)

    WB analysis using 84576-4-RR (same clone as 84576-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84576-4-RR (MDH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84576-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 84576-4-RR (same clone as 84576-4-PBS)

    IHC staining of mouse heart using 84576-4-RR (same clone as 84576-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84576-4-RR (MDH1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84576-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 84576-4-RR (same clone as 84576-4-PBS)

    IHC staining of mouse heart using 84576-4-RR (same clone as 84576-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84576-4-RR (MDH1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84576-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84576-4-RR

    Affinity and Kinetic Characterization of 84576-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84576-4-RR against Human MDH1 were performed. The affinity constant is below 1 pM.

MDH1 Recombinant antibody, PBS Only

MDH1 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84576-4-PBS

产品说明书

CloneNo. 241887A1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

MDHA, KAR, EC:1.1.1.96, EC:1.1.1.37, Aromatic alpha-keto acid reductase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84576-4-PBS targets MDH1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MDH1 fusion protein Ag8744 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 malate dehydrogenase 1, NAD (soluble)
别名 MDHA, KAR, EC:1.1.1.96, EC:1.1.1.37, Aromatic alpha-keto acid reductase
计算分子量 334 aa, 36 kDa
观测分子量 35-37 kDa
GenBank蛋白编号BC001484
基因名称 MDH1
Gene ID (NCBI) 4190
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP40925
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme.(PMID:10693743)