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MDH1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.781-100 ng/mL

Sandwich ELISA: 0.391-25 ng/mL

Cat No : MP01422-2

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Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01422-2, Human MDH1 Recombinant Matched Antibody Pair - PBS only. 84576-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag8744. 84576-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

    Sandwich ELISA standard curve of MP01422-2

    Sandwich ELISA standard curve of MP01422-2, Human MDH1 Recombinant Matched Antibody Pair - PBS only. 84576-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag8744. 84576-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

  • Serum of six individual healthy human donors was measured. The MDH1 concentration of detected samples was determined to be 92.63 ng/mL with a range of 59.50-142.37 ng/mL.

    Sample test of MP01422-2

    Serum of six individual healthy human donors was measured. The MDH1 concentration of detected samples was determined to be 92.63 ng/mL with a range of 59.50-142.37 ng/mL.

  • The mean MDH1 concentration was determined to be 2187.75 ng/mL in HepG2 cell extract based on a 2.30 mg/mL extract load and 1287.87 ng/mL in HEK-293 cell extract based on a 1.50 mg/mL extract load.

    Sample test of MP01422-2

    The mean MDH1 concentration was determined to be 2187.75 ng/mL in HepG2 cell extract based on a 2.30 mg/mL extract load and 1287.87 ng/mL in HEK-293 cell extract based on a 1.50 mg/mL extract load.

  • Cytometric bead array standard curve of MP01422-2, MDH1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84576-1-PBS. Detection antibody: 84576-2-PBS. Standard: Ag8744. Range: 0.781-100 ng/mL.

    Cytometric bead array standard curve of MP01422-2

    Cytometric bead array standard curve of MP01422-2, MDH1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84576-1-PBS. Detection antibody: 84576-2-PBS. Standard: Ag8744. Range: 0.781-100 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP01422-2 targets MDH1 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.781-100 ng/mL (Cytometric Bead Array); 0.391-25 ng/mL (Sandwich ELISA);

Capture antibody: MDH1 Recombinant antibody, PBS Only (Capture/Detector) 84576-1-PBS (241887C5). 100 μg. Concentration 1 mgl/ml.

Detection antibody: MDH1 Recombinant antibody, PBS Only (Detector) 84576-2-PBS (241887D8). 100 μg. Concentration 1 mgl/ml.

Alternative MDH1 matched antibody pairs: MP01422-1

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84576-1-PBS
Tested Reactivity human
Immunogen MDH1 fusion protein Ag8744
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name malate dehydrogenase 1, NAD (soluble)
Synonyms MDHA, KAR, EC:1.1.1.96, EC:1.1.1.37, Aromatic alpha-keto acid reductase
GenBank accession numberBC001484
Gene symbol MDH1
UniprotP40925
Gene ID (NCBI) 4190
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 84576-2-PBS
Tested Reactivity human
Immunogen MDH1 fusion protein Ag8744
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name malate dehydrogenase 1, NAD (soluble)
Synonyms MDHA, KAR, EC:1.1.1.96, EC:1.1.1.37, Aromatic alpha-keto acid reductase
GenBank accession numberBC001484
Gene symbol MDH1
UniprotP40925
Gene ID (NCBI) 4190
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C