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MDMX/MDM4 Polyclonal antibody

MDMX/MDM4 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 28747-1-AP

Print datasheet

Synonyms

MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX

验证数据展示

  • WB analysis using 28747-1-AP

    WB analysis using 28747-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28747-1-AP (MDMX antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 28747-1-AP

    IHC staining of human ovary tumor using 28747-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28747-1-AP (MDM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of MCF-7 using 28747-1-AP

    IP experiment of MCF-7 using 28747-1-AP

    IP result of anti-MDMX (IP:28747-1-AP, 4ug; Detection:28747-1-AP 1:3000) with MCF-7 cells lysate 1470 ug.

  • IHC staining of human breast cancer using 28747-1-AP

    IHC staining of human breast cancer using 28747-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28747-1-AP (MDM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 28747-1-AP

    IHC staining of human ovary tumor using 28747-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28747-1-AP (MDMX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA2780 cells, MCF-7 cells, MDA-MB-231 cells, U2OS cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28747-1-AP targets MDMX/MDM4 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen MDMX/MDM4 fusion protein Ag30456 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Mdm4 p53 binding protein homolog (mouse)
Synonyms MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX
Calculated Molecular Weight 490 aa, 55 kDa
Observed Molecular Weight 70-76 kDa
GenBank Accession NumberBC067299
Gene Symbol MDMX/MDM4
Gene ID (NCBI) 4194
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15151
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kDa, while the observed mass is 70-80 kDa, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370, 28097652). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for MDMX/MDM4 antibody 28747-1-APDownload protocol
IHC protocol for MDMX/MDM4 antibody 28747-1-APDownload protocol
IP protocol for MDMX/MDM4 antibody 28747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Sci

SNRPB2 promotes triple-negative breast cancer progression by controlling alternative splicing of MDM4 pre-mRNA

Authors - Shiyi Yu
View Article