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验证数据展示

  • FC experiment of HeLa using 68117-1-Ig

    FC experiment of HeLa using 68117-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug ME1 Monoclonal antibody (68117-1-Ig, Clone:2G9G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 68117-1-Ig

    WB analysis using 68117-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68117-1-Ig (ME1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68117-1-Ig

    WB analysis using 68117-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68117-1-Ig (ME1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 68117-1-Ig

    IF Staining of HepG2 using 68117-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ME1 antibody (68117-1-Ig, Clone: 2G9G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

ME1 Monoclonal antibody

ME1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 68117-1-Ig

产品说明书

CloneNo. 2G9G9
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IF/ICC, FC (Intra), ELISA

别名

NADP-ME, NADP-dependent malic enzyme, Malic enzyme 1, EC:1.1.1.40, 2G9G9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, pig brain tissue, HCT 116 cells, LNCaP cells, HeLa cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68117-1-Ig targets ME1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat, pig, rabbit
文献引用反应性human
免疫原 ME1 fusion protein Ag9916 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 malic enzyme 1, NADP(+)-dependent, cytosolic
别名 NADP-ME, NADP-dependent malic enzyme, Malic enzyme 1, EC:1.1.1.40, 2G9G9
计算分子量 572 aa, 64 kDa
观测分子量55-64 kDa
GenBank蛋白编号BC025246
基因名称 ME1
Gene ID (NCBI) 4199
RRIDAB_2923646
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP48163
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ME1(NADP-dependent malic enzyme) belongs to the malic enzymes family. This malic enzyme catalyzes the reversible oxidative decarboxylation of malate and is a link between the glycolytic pathway and the citric acid cycle. The reaction is L-malate plus NADP(+) to form pyruvate, CO(2), and NADPH.

实验方案

Product Specific Protocols
WB protocol for ME1 antibody 68117-1-IgDownload protocol
IF protocol for ME1 antibody 68117-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Ther Adv Hematol

High expression of malic enzyme 1 predicts adverse prognosis in patients with cytogenetically normal acute myeloid leukaemia and promotes leukaemogenesis through the IL-6/JAK2/STAT3 pathways

Authors - Chang Zhang
  • KD Validated
View Article