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MEA1 Polyclonal antibody

MEA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10267-1-AP

Print datasheet

Synonyms

HYS, male enhanced antigen 1, MEA, MEA 1, MEA1

验证数据展示

  • WB analysis of Jurkat using 10267-1-AP

    WB analysis of Jurkat using 10267-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10267-1-AP (MEA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 10267-1-AP

    IHC staining of human pancreas cancer using 10267-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10267-1-AP (MEA1 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inJurkat cells, mouse testis tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10267-1-AP targets MEA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MEA1 fusion protein Ag0375 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name male-enhanced antigen 1
Synonyms HYS, male enhanced antigen 1, MEA, MEA 1, MEA1
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 34 kDa and 36 kDa
GenBank Accession NumberBC001754
Gene Symbol MEA1
Gene ID (NCBI) 4201
RRIDAB_2143838
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16626
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEA1, also named Male-enhanced antigen 1, is a 185 amino acid protein, which is highly expressed in testis. MEA1 may play an important role in spermatogenesis and/or testis development. Endogenous protein was detected as two bands (34 kDa and 36 kDa). (PMID: 12444059)

实验方案

Product Specific Protocols
WB protocol for MEA1 antibody 10267-1-APDownload protocol
IHC protocol for MEA1 antibody 10267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols