• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MED12L Polyclonal antibody

MED12L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20047-1-AP

Print datasheet

Synonyms

TNRC11L, Thyroid hormone receptor-associated-like protein, PRO0314, Mediator of RNA polymerase II transcription subunit 12-like protein, Mediator complex subunit 12-like protein

验证数据展示

  • WB analysis of mouse liver using 20047-1-AP

    WB analysis of mouse liver using 20047-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20047-1-AP (MED12L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20047-1-AP targets MED12L in WB, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mediator complex subunit 12-like
Synonyms TNRC11L, Thyroid hormone receptor-associated-like protein, PRO0314, Mediator of RNA polymerase II transcription subunit 12-like protein, Mediator complex subunit 12-like protein
Calculated Molecular Weight 240 kDa
Observed Molecular Weight 260-280 kDa
GenBank Accession NumberNM_053002
Gene Symbol MED12L
Gene ID (NCBI) 116931
RRIDAB_10734594
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86YW9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED12L, also named as KIAA1635, TNRC11L, TRALP and TRALPUSH, belongs to the Mediator complex subunit 12 family. MED12L may be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED12L is a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED12L is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The antibody can recognize all the isoform of MED12L. The antibody has no cross reaction to MED12.

实验方案

Product Specific Protocols
WB protocol for MED12L antibody 20047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols