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MED23 Polyclonal antibody

MED23 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27850-1-AP

Print datasheet

Synonyms

MED23/CRSP3, CRSP133, CRSP130, CRSP complex subunit 3, Cofactor required for Sp1 transcriptional activation subunit 3

验证数据展示

  • WB analysis of A375 using 27850-1-AP

    WB analysis of A375 using 27850-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 27850-1-AP (MED23 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 27850-1-AP

    WB analysis using 27850-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27850-1-AP (MED23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 27850-1-AP

    IHC staining of human stomach using 27850-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27850-1-AP (MED23 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inSH-SY5Y cells, A375 cells, U2OS cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27850-1-AP targets MED23 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen MED23 fusion protein Ag27241 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mediator complex subunit 23
Synonyms MED23/CRSP3, CRSP133, CRSP130, CRSP complex subunit 3, Cofactor required for Sp1 transcriptional activation subunit 3
Calculated Molecular Weight 156 kDa
Observed Molecular Weight 140 kDa, 50 kDa
GenBank Accession NumberBC060759
Gene Symbol MED23
Gene ID (NCBI) 9439
RRIDAB_2880994
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULK4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED23 is a component of the Mediator complex, it is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.

实验方案

Product Specific Protocols
WB protocol for MED23 antibody 27850-1-APDownload protocol
IHC protocol for MED23 antibody 27850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Rep

DDB1 binds histone reader BRWD3 to activate the transcriptional cascade in adipogenesis and promote onset of obesity.

Authors - Xu Wang
View Article