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MED28 Polyclonal antibody

MED28 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16262-1-AP

Print datasheet

Synonyms

1500003D12Rik, EG1, Endothelial derived protein 1, magicin, MED28, mediator complex subunit 28, Tumor angiogenesis marker EG 1

验证数据展示

  • WB analysis using 16262-1-AP

    WB analysis using 16262-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16262-1-AP (MED28 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 16262-1-AP

    IHC staining of mouse testis using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 16262-1-AP

    IHC staining of mouse testis using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue, PC-12 cells, rat testis tissue
Positive IHC detected inmouse testis tissue, mouse brain tissue, human kidney tissue, human placenta tissue, human testis tissue, human brain tissue, human liver tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16262-1-AP targets MED28 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MED28 fusion protein Ag9327 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mediator complex subunit 28
Synonyms 1500003D12Rik, EG1, Endothelial derived protein 1, magicin, MED28, mediator complex subunit 28, Tumor angiogenesis marker EG 1
Calculated Molecular Weight 178 aa, 20 kDa
Observed Molecular Weight24 kDa
GenBank Accession NumberBC011936
Gene Symbol MED28
Gene ID (NCBI) 80306
RRIDAB_2281863
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H204
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MED28 antibody 16262-1-APDownload protocol
IHC protocol for MED28 antibody 16262-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols