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验证数据展示

  • WB analysis using 16262-1-AP

    WB analysis using 16262-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16262-1-AP (MED28 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 16262-1-AP

    IHC staining of mouse testis using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 16262-1-AP

    IHC staining of mouse testis using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MED28 Polyclonal antibody

MED28 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16262-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

1500003D12Rik, EG1, Endothelial derived protein 1, magicin, MED28, mediator complex subunit 28, Tumor angiogenesis marker EG 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, PC-12 cells, rat testis tissue
Positive IHC detected inmouse testis tissue, mouse brain tissue, human kidney tissue, human placenta tissue, human testis tissue, human brain tissue, human liver tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16262-1-AP targets MED28 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MED28 fusion protein Ag9327 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mediator complex subunit 28
别名 1500003D12Rik, EG1, Endothelial derived protein 1, magicin, MED28, mediator complex subunit 28, Tumor angiogenesis marker EG 1
计算分子量 178 aa, 20 kDa
观测分子量24 kDa
GenBank蛋白编号BC011936
基因名称 MED28
Gene ID (NCBI) 80306
RRIDAB_2281863
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H204
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MED28 antibody 16262-1-APDownload protocol
IHC protocol for MED28 antibody 16262-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols