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  • Featured Product
  • KD/KO Validated

MED30 Polyclonal antibody

MED30 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16787-1-AP

Print datasheet

Synonyms

MED30, mediator complex subunit 30, THRAP6, TRAP25

验证数据展示

  • WB analysis of human heart using 16787-1-AP

    WB analysis of human heart using 16787-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16787-1-AP (MED30 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse heart using 16787-1-AP

    IP experiment of mouse heart using 16787-1-AP

    IP result of anti-MED30 (IP:16787-1-AP, 4ug; Detection:16787-1-AP 1:500) with mouse heart tissue lysate 4000ug.

  • WB analysis of HEK-293 using 16787-1-AP

    WB analysis of HEK-293 using 16787-1-AP

    WB result of MED30 antibody (16787-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MED30 transfected HEK-293 cells.

经过测试的应用

Positive WB detected inhuman heart tissue, HEK-293 cells
Positive IP detected inmouse heart tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16787-1-AP targets MED30 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen MED30 fusion protein Ag10221 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mediator complex subunit 30
Synonyms MED30, mediator complex subunit 30, THRAP6, TRAP25
Calculated Molecular Weight 178 aa, 20 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC008226
Gene Symbol MED30
Gene ID (NCBI) 90390
RRIDAB_2142439
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96HR3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED30, also named as THRAP6 and TRAP25, is a component of the Mediator complex. MED30 has two isoforms with MW 16-20 kDa ad 20-25 kDa.

实验方案

Product Specific Protocols
WB protocol for MED30 antibody 16787-1-APDownload protocol
IP protocol for MED30 antibody 16787-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

J Autoimmun

Immune complexome analysis of a rich variety of serum immune complexes identifies disease-characteristic immune complex antigens in systemic sclerosis

Authors - Yuki Jimbayashi Kutsuna
View Article
humanWB, IHC

PLoS One

MED30 Regulates the Proliferation and Motility of Gastric Cancer Cells.

Authors - Yong Joo Lee
View Article
humanWB,IHC

Anticancer Res

The Contrasting Role of the Mediator Subunit MED30 in the Progression of Bladder Cancer.

Authors - Isabella Syring
  • KD Validated
View Article
humanWB,IHC

Ann Diagn Pathol

The knockdown of the mediator complex subunit MED30 suppresses the proliferation and migration of renal cell carcinoma cells.

Authors - Isabella Syring
  • KD Validated
View Article