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MED31 Polyclonal antibody

MED31 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16590-1-AP

Print datasheet

Synonyms

SOH1, Mediator of RNA polymerase II transcription subunit 31, hSOH1, CGI-125

验证数据展示

  • WB analysis of mouse testis using 16590-1-AP

    WB analysis of mouse testis using 16590-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16590-1-AP (MED31 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16590-1-AP

    IF Staining of HepG2 using 16590-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MED31 antibody (16590-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse testis tissue, human brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16590-1-AP targets MED31 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen MED31 fusion protein Ag9008 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mediator complex subunit 31
Synonyms SOH1, Mediator of RNA polymerase II transcription subunit 31, hSOH1, CGI-125
Calculated Molecular Weight 131 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC012539
Gene Symbol MED31
Gene ID (NCBI) 51003
RRIDAB_2142447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3C7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED31, also named as SOH1 or CGI-125, is a 131 amino acid protein, which belongs to the Mediator complex subunit 31 family. MED31 localizes in the nucleus. MED31 as a component of the Mediator complex, a coactivator is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED31 functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.

实验方案

Product Specific Protocols
WB protocol for MED31 antibody 16590-1-APDownload protocol
IF protocol for MED31 antibody 16590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Dev Biol

The Mediator complex protein Med31 is required for embryonic growth and cell proliferation during mammalian development.

Authors - Risley Michael D MD
View Article