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MED6 Polyclonal antibody

MED6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15338-1-AP

Print datasheet

Synonyms

ARC33, hMed6, MED6, mediator complex subunit 6, NY REN 28

验证数据展示

  • WB analysis using 15338-1-AP

    WB analysis using 15338-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15338-1-AP (MED6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 15338-1-AP

    IHC staining of human prostate cancer using 15338-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15338-1-AP (MED6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells, Jurkat cells, HepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15338-1-AP targets MED6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MED6 fusion protein Ag7541 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mediator complex subunit 6
Synonyms ARC33, hMed6, MED6, mediator complex subunit 6, NY REN 28
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-32 kDa
GenBank Accession NumberBC004106
Gene Symbol MED6
Gene ID (NCBI) 10001
RRIDAB_2142554
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75586
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

实验方案

Product Specific Protocols
WB protocol for MED6 antibody 15338-1-APDownload protocol
IHC protocol for MED6 antibody 15338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols