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验证数据展示

  • WB analysis using 68352-1-Ig

    WB analysis using 68352-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68352-1-Ig (MED6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

MED6 Monoclonal antibody

MED6 Monoclonal Antibody for WB, ELISA
Cat No. 68352-1-Ig

Print datasheet

CloneNo. 3E4G10

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

ARC33, hMed6, MED6, mediator complex subunit 6, NY REN 28

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, T-47D cells, SK-BR-3 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, 4T1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68352-1-Ig targets MED6 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen MED6 fusion protein Ag7645 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name mediator complex subunit 6
Synonyms ARC33, hMed6, MED6, mediator complex subunit 6, NY REN 28
Calculated Molecular Weight 28 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC004106
Gene Symbol MED6
Gene ID (NCBI) 10001
RRIDAB_3085075
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75586
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

实验方案

Product Specific Protocols
WB protocol for MED6 antibody 68352-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols