验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, LNCaP cells, HeLa cells, T-47D cells, SK-BR-3 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, 4T1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68352-1-Ig targets MED6 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | MED6 fusion protein Ag7645 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | mediator complex subunit 6 |
| 别名 | ARC33, hMed6, MED6, mediator complex subunit 6, NY REN 28 |
| 计算分子量 | 28 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC004106 |
| 基因名称 | MED6 |
| Gene ID (NCBI) | 10001 |
| RRID | AB_3085075 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O75586 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MED6 antibody 68352-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |