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MED8 Polyclonal antibody

MED8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12182-1-AP

Print datasheet

Synonyms

ARC32, MED8, mediator complex subunit 8

验证数据展示

  • IHC staining of human brain using 12182-1-AP

    IHC staining of human brain using 12182-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12182-1-AP (MED8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 12182-1-AP

    IHC staining of human brain using 12182-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12182-1-AP (MED8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HepG2 using 12182-1-AP

    WB analysis of HepG2 using 12182-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, mouse liver tissue, SGC-7901 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12182-1-AP targets MED8 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen MED8 fusion protein Ag2827 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mediator complex subunit 8
Synonyms ARC32, MED8, mediator complex subunit 8
Calculated Molecular Weight 212 aa, 23 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC010543
Gene Symbol MED8
Gene ID (NCBI) 112950
RRIDAB_2877834
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96G25
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED8, a component of the Mediator complex, is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. It plays a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. MED8 has three isoforms with MW 19 kDa, 29 kDa and 33 kDa. And the ubiquitination form will be 38-43 kDa.

实验方案

Product Specific Protocols
WB protocol for MED8 antibody 12182-1-APDownload protocol
IHC protocol for MED8 antibody 12182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Rep

The Mediator subunit MED20 organizes the early adipogenic complex to promote development of adipose tissues and diet-induced obesity.

Authors - Wen-Shuai Tang
View Article