• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HEK-293T using 27452-1-AP

    WB analysis of HEK-293T using 27452-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27452-1-AP (MED9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

MED9 Polyclonal antibody

MED9 Polyclonal Antibody for WB, ELISA
Cat No. 27452-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MED25, MED9, mediator complex subunit 9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293T cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27452-1-AP targets MED9 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen MED9 fusion protein Ag26158 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mediator complex subunit 9
Synonyms MED25, MED9, mediator complex subunit 9
Calculated Molecular Weight 146 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC000647
Gene Symbol MED9
Gene ID (NCBI) 55090
RRIDAB_2880873
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWA0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED9, also named as MED25, is a 146 amino acid protein, which belongs to the Mediator complex subunit 9 family. MED9 is a component of the Mediator complex and a coactivator that is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED9 as a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.

实验方案

Product Specific Protocols
WB protocol for MED9 antibody 27452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols