验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells, SH-SY5Y cells, THP-1 cells |
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
28819-1-AP targets MEF2A in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | MEF2A fusion protein Ag30349 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myocyte enhancer factor 2A |
| 别名 | ADCAD1, MEF2, MEF2A, myocyte enhancer factor 2A, RSRFC4, RSRFC9 |
| 计算分子量 | 499 aa, 54 kDa |
| 观测分子量 | 50-70kDa |
| GenBank蛋白编号 | BC013437 |
| 基因名称 | MEF2A |
| Gene ID (NCBI) | 4205 |
| RRID | AB_3086086 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q02078 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MEF2A belongs to a family of DNA binding regulatory proteins. Each of these proteins which belong to the myocyte-specific enhancer factor-2 (MEF2) family binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. MEF2A plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. MEF2A also has key roles in cardiac and skeletal muscle development. MEF2A is especially abundant in granule neurons of the cerebellar cortex throughout the period of synaptogenesis. This antibody can detect two bands around 60 kDa to 70 kDa, the larger band may correspond to its post-translational modifications.(PMID: 15483225,16484498, 23486945 )
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MEF2A antibody 28819-1-AP | Download protocol |
| IHC protocol for MEF2A antibody 28819-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
