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验证数据展示

  • WB analysis of human heart using 16953-1-AP

    WB analysis of human heart using 16953-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16953-1-AP (MEF2C antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of TF-1 using 16953-1-AP

    WB analysis of TF-1 using 16953-1-AP

    TF-1 cells were subjected to SDS PAGE followed by western blot with 16953-1-AP (MEF2C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

MEF2C Polyclonal antibody

MEF2C Polyclonal Antibody for WB, ELISA
Cat No. 16953-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

myocyte enhancer factor 2C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue, HeLa cells, mouse skeletal muscle tissue, TF-1 cells
Planning an IHC experiment? We recommend our IHCeasy MEF2C Ready-To-Use IHC Kit. MEF2C primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16953-1-AP targets MEF2C in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MEF2C fusion protein Ag10583 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myocyte enhancer factor 2C
别名 myocyte enhancer factor 2C
计算分子量 469 aa, 51 kDa
观测分子量45-70 kDa
GenBank蛋白编号BC026341
基因名称 MEF2C
Gene ID (NCBI) 4208
RRIDAB_1851364
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ06413
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-66 kDa.

实验方案

Product Specific Protocols
WB protocol for MEF2C antibody 16953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols