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MEF2C Polyclonal antibody

MEF2C Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16953-1-AP

Print datasheet

Synonyms

myocyte enhancer factor 2C

验证数据展示

  • WB analysis of human heart using 16953-1-AP

    WB analysis of human heart using 16953-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16953-1-AP (MEF2C antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of TF-1 using 16953-1-AP

    WB analysis of TF-1 using 16953-1-AP

    TF-1 cells were subjected to SDS PAGE followed by western blot with 16953-1-AP (MEF2C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman heart tissue, HeLa cells, mouse skeletal muscle tissue, TF-1 cells
Planning an IHC experiment? We recommend our IHCeasy MEF2C Ready-To-Use IHC Kit. MEF2C primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16953-1-AP targets MEF2C in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MEF2C fusion protein Ag10583 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myocyte enhancer factor 2C
Synonyms myocyte enhancer factor 2C
Calculated Molecular Weight 469 aa, 51 kDa
Observed Molecular Weight45-70 kDa
GenBank Accession NumberBC026341
Gene Symbol MEF2C
Gene ID (NCBI) 4208
RRIDAB_1851364
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ06413
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-66 kDa.

实验方案

Product Specific Protocols
WB protocol for MEF2C antibody 16953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols